The floating original medium was collected and the cells were digested with 0.25% trypsin without EDTA (Hyclone; GE Healthcare Existence Sciences) at 37C for 1 min. were recognized by a Cell Counting-Kit 8 assay and circulation cytometry, respectively. In the shKEAP1 Hep2 cell collection, the mRNA and protein manifestation levels of NRF2, NQO1 and HO1 … Continue reading The floating original medium was collected and the cells were digested with 0